Despite a strong opening 45 minutes, Dons went in level after Anthony Scully’s penalty cancelled out Stephen Wearne’s opener - the first goal on the road this season for Mike Williamson’s side.
A Fiacre Kelleher strike nine minutes after half-time then secured the points for Colchester.
Here’s how we rated the players
13. Sub: Tommy Leigh - 5.5
Had a decent shot saved but otherwise struggled to get into the game | Jane Russell
14. Sub: Rian Silver - 6.5
Looked confident and assured on the ball in the centre of the park | Jane Russell
15. Sub: Keon Lewis-Burgess - 6
Slotted into the defence with ease, and distributed well | Jane Russell
16. Sub: Connor Lemonheigh-Evans - 5.5
Just like in his debut against Colchester, came on late out wide, and missed a good header in stoppage time | Jane Russell
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